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Cells were maintained in a humidified environment of 5% CO 2 at 37 C, cultured until an optical confluence of 75-100%, detached with 0.05% trypsin-EDTA 1 (25300-054, Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA), at 37 C for approximately 5 min, and split to new plates according to the experimental procedures
Is headache a sign that GLP-1 medication is working
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Short-term side effects include insomnia (take morning dose), increased appetite, mood changes, elevated blood sugar, and temporary blood pressure increasesinform the provider if you have diabetes or hypertension
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