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Only as prescribed by a healthcare professional, usually in clinical or hospital settings

Animals were humanely exposed to cervical dislocation after anesthesia by IP injection of 0.1 ml / 100 kg /rat from the mixture of ketamine (90 mg/kg) and xylazine (5 mg/kg)

The concentration of complementary deoxyribonucleic acid (cDNA) after reverse transcription were also determined using NanoDrop Spectrophotometer