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Because accurate determination of the NADPH consumption rate can be confounded by the reduction rate of NADP + , we inhibited key biochemical pathways that reduce NADP + back to NADPH in LPS-stimulated Ms, including glucose-6-phosphate dehydrogenase (G6PD), isocitrate dehydrogenases and folate-mediated one carbon metabolism that can contribute to the reduction of NADP + [1820]
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At the same time, increased levels of inflammatory molecules, such as LPS from harmful bacteria, can lead to systemic-wide inflammation (Obrenovich et al., 2020)
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