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Elevated intracellular calcium concentrations, in turn, appear to prompt the secretory vesicles inside these cells to release growth hormone into the bloodstream
It differs from native IGF-1 in that it possesses an arginine instead of a glutamic acid at the third position in its amino acid sequence, and also has an additional 13 amino acids at its N-terminus (MFPAMPLLSLFVN), for a total of 83 amino acids relative to the 70 of native IGF-1
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Briefly, cells were seeded in six-well plate, growth media was removed from the cells before assay followed by a rinse with PBS
This is because they target different stages of the anabolic process with HGH acting as the initiator and IGF-1 functioning as the executioner of muscle growth and repair
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