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These signaling mechanisms increase aromatase activity and LHR expression (60)

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During GLP-1R blockade, infusion of glucose was immediately required upon starting GLP-1m infusions in all subjects due to a more dramatic reduction in HGP, as well as a delayed and modest insulinotropic response

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The expression of each gene of interest (Supplementary Table 1) was assessed using the Comparative CT method employing SYBR green master mix (Invitrogen Cat #4309155)