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doi: 10.3390/nu13103519 44 ZamboniMMazzaliGBrunelliASaatchiTUrbaniSGianiAet al

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Extracts of the medium were incubated at 80 C for at least 15 min before being centrifuged at 15,000 g for 20 min

Factors like better quality of life, better diagnostic tools and screening technology, enhanced medical intervention availability, and preventive medication like vaccines, amongst others, served a crucial role in the decline in the numbers