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Then, the medium was changed to fresh DMEM containing DCFH-DA (10 M) and Hoechst 33342 for ROS analysis and nuclei stain

These reactions are usually mild, short-lived, and resolve without medical intervention

These conditions can be fatal, but theyre also treatable

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One are those which are dark due to under eye bags and loss of volume and others are dark without loss of volume

Neither of the mutants showed a decrease in virulence, indicating that the Bcgst1 gene is not essential for virulence on tomato leaves under the conditions tested (Prins et al., 2000)