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Anemia is diagnosed using the following methods: A complete blood count (CBC): The CBC measures the number, size, and shape of red blood cells, as well as the hemoglobin level and hematocrit (Hct%) in red blood cells, white blood cells, and platelets, including the levels of vitamin B12 and vitamin B9 (folate) in whole blood

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The in vitro experiments were performed using TM3 mouse Leydig cells that were cultured in DMEM/F-12 medium supplemented with 5% horse serum and 2.5% fetal bovine serum, and maintained at 37C under a 5% CO2 atmosphere

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it's the evidence-based threshold where degradation becomes statistically significant