Bartolini et al., 2021)
PI3K/Akt/mTOR signaling pathway: role in esophageal squamous cell carcinoma, regulatory mechanisms and opportunities for targeted therapy
First, convert milligrams (mg) to micrograms (mcg): 10mg = 10,000mcg
The compound is supplied as a blue to blue-purple lyophilized powder, highly soluble in water, and should be stored refrigerated at 36-46 degrees F (2-8 degrees C) and protected from light
linear detection range 0.4 to 50 U/L with a 20 uL sample, detection limit 0.4 U/L Convenient and high-throughput mix-incubate-measure format Readily automated on liquid handling systems Assays can be run at any desired temperature, such as 25C or 37C Glutathione reductase activity determination in biological samples such as plasma, serum, tissue and culture media Note: The below protocol is a sample protocol
Glutathione depletion is accompanied by increased neuronal nitric oxide synthase activity