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In a co-administered protocol the three components depopulate at different rates from a single bolus, which is one of the legitimate criticisms of any fixed-ratio blend: the researcher cannot independently titrate frequency for the shorter-half-life components without also re-dosing the longer-half-life one

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The same observations were made for treatment of GSSG in the presence of iron(II) complex ( 1 ) and iron(III) complex ( 3 ), both resulting in the immediate loss of GSSG signal intensity