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24 h later, culture media were replaced with 1 mL of serum-free DMEM containing 50 ng/mL of recombinant hTNF- (Peprotech) and cultured for a further 24 h before collecting cells and supernatants

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6 Future prospects and or research directions into improving rooster semen quality 6.1 Optimizing LCPUFA supplementation for enhancing reproductive performance in roosters The balance between 3 and 6 fatty acids in the diet is vital (Jeong et al., 2024)

Gliotoxin is also the only toxin that has been isolated from the sera of patients with invasive aspergillosis

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The expression of rHrGSTm1 was the highest in the supernatant of bacteria induced by 0.5 mM IPTG for 8 h at 37 C, and a single band of rHrGSTm1 was obtained by elution with 500 mm imidazole (Fig