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Control cells were treated with H 2 O 2 (1 mM) for 30 min as a positive control for increased ROS production

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As IKK- is the direct upstream kinase that phosphorylates IB-, leading to its degradation, we next examined whether decreased TNF-induced IB- phosphorylation by H 2 O 2 treatment was due to its effects on IKK- activity

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