After centrifugation at 15,000 g for 50 min, the cell supernatant was incubated with GST beads (Cytiva, Glutathione Sepharose 4B, 17075605) at 4 C for 1.52 h
Timing, dosing, and routine all play a role in how effectively the body responds
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We chose to focus on SLC7A11 as playing a critical role in intracellular GSH levels, targeting SLC7A11 with erastin
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