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Glutathione- and glutaredoxin-dependent reduction of methionine sulfoxide reductase A
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Cell-free glutathionylation experiments Fifty ng of recombinant IL-1sRII (R&D systems) or recombinant Lyn purified from HEK293T cells were treated with 100 mM of DTT for 20 min at room temperature to reduce free cysteines
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