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The physiological basis of our model is the reaction diagram [Figure 1] and the K m values for the enzymes

h j , Exponential-phase cultures were treated with KL1 (40 M) or DMSO (0.1%) for 4 h, followed by measuring relative ATP levels

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doi: 10.1021/jacs.0c12322 30 KoikeSYamasakiK

After pooling the fractions containing pure 3,4- cis -leucocyanidin ( 6 ), MOPSO was added to a final concentration of 10 mM, the pH was set to 6.2 using 5 N NaOH and the pooled fractions were rotary-evaporated to a concentration of 7 mM of 3,4- cis -leucocyanidin ( 6 ), quantified by HPLC-UV (280 nm) with an external calibration curve with 3,4- trans -leucocyanidin ( 14 )

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