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Reverse transcription and quantitative PCR (RT-qPCR) Total RNA was extracted using the RNeasy Mini Extraction kit (74104, Qiagen, Venlo, Netherlands), according to the manufacturers protocol

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The utilization of in vitro models becomes an even more appealing option if we take into account the growing ethical constraints on the use of animals and human samples in biomedical research

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5A, the crude protein level in the carnitine supplementation group was significantly increased compared with the control group in the feeding state, but was significantly decreased in the fasting state (Fig

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