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These sections were immediately placed in primary fixative solution (2% formaldehyde, 2.5% glutaraldehyde, and 2 mM CaCl 2 in 0.15 M cacodylate buffer), 1 mm 3 pieces were then transferred into a new fixative solution for 3 h at room temperature, washed three times with 0.15 M cacodylate buffer and then post-fixed and contrasted using a modified OTO protocol (2% osmium tetroxide + 1.5% potassium ferricyanide-(II), thiocarbohydrazide, 2% osmium tetroxide, 2% uranyl acetate) 74

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