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The lower chamber was added with various materials supplemented with 10% FBS, while serum-free DMEM was added to the upper chamber
Kidney conditions may reduce the kidneys ability to filter and clear vitamin B12 and its binding proteins, also leading to higher measured levels
It's sterile but lacks bacteriostatic properties
Retrieved 2019-03-09
HFF-1 cells maintained in a T-25 cell culture flask (Corning, United States) at 37C in a 5% (v/v) CO 2 atmosphere and harvested at 90% confluence using 0.12% trypsin-EDTA
Funding This study was supported by Grant-in-Aid for Young Researchers from Saitama Medical University International Medical Center (4-D-1-04) (MH)
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