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19) was added at 10 M into the medium for 24 hours before harvest, with dimethyl sulfoxide or water added as vehicle control

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Month-over-month ADM prescribing continued to increase with time

Protein in sample A and B was removed by precipitating with 200 L of cold ACN (containing 1 g/mL carbachol, the internal standard, IS) and centrifuging at 16,900 g for 20 min

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