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This phenomenon may be partly explained by biological differences such as variations in sex hormone levels, pancreatic -cell composition, and gene expression patterns related to insulin secretion and glucose metabolism [49]

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It should be noted that an increase in the dose of L-carnitine from 200 mg/kg to 800 mg/kg was not accompanied by a significant increase in the therapeutic effect

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After a further centrifugation (200 x g, 8 min, 20C, Universal 320, Andreas Hettich GmbH, Tuttlingen, Germany), PBMC were resuspended in RPMI medium supplemented with fetal bovine serum, HEPES buffer, L-glutamine, and -mercaptoethanol (all purchased from Biochrom AG, Berlin, Germany)

The most frequent disorder in human is the glutathione synthetase (GSS) deficiency, which is often associated with hemolytic anemia, a clinical feature also present in -glutamylcysteine (GCLC) deficiency