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RNA interference To silence GLP-1R and PKA gene expression, cells were transfected with siRNAs using lipofectamine RNAi MAX reagent (Invitrogen)
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The concentrations of glucose and acetic acid were monitored by HPLC (LC-10AT, Shimadzu, Japan) using an Amines HPX-87H column (Bio-Rad, USA) and a refractive index detector (RID-10A, Shimadzu, Japan)
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