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PVN-cannulated Glp1r OBhM3Dq mice were PVN-infused with Bicuculline Methiodide (Sigma-Aldrich

Puncture the rubber stopper of the glutathione vial with the syringe

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Peptide hormones work by binding to specific receptors on the surface of target cells, initiating a cascade of intracellular events that result in the desired biological effect