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[157] Isotretinoin, a vitamin A analog, is prescribed for keratinization disorders, severe nodular acne, and psoriasis, but its use is connected to various ocular manifestations
Huppke P, Weissbach S, Church JA, Schnur R, Krusen M, Dreha-Kulaczewski S, et al
Step 5 : 4 th Chromatography step Fig.5
Thiol-containing compounds (RSH) react with DTNB to form mixed disulfides (RS-S-DTNB), where the thiol group replaces one of the nitrobenzoic acid moieties in DTNB
& Altszuler, N