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It's not so intimidating when you break it down
Every vial ships with an HPLC purity trace (often 99%), mass-spec identity confirmation, and endotoxin/heavy-metal screening on the batch
The next day, the membrane was incubated overnight at 4 C with 350 nM human recombinant XPO1 and 1050 nM yeast recombinant GSP1 (RAN1 ortholog) in 2.5% BSA and XPO1 purification buffer