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Tubes were centrifuged at 300 g for 5 min at 4 C to collect TIF
This relatively high concentration helps protect the stability of the red blood cell membrane and prevents hemoglobin from being oxidized to methemoglobin, thereby preserving its oxygen-carrying capacity
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Ethics declarations Funding The open access fee was funded by Nicolaus Copernicus University in Torun
Therefore, the subsequent breakdown of such proteins results in the release of methylated arginine derivatives: NG-monomethyl-L-arginine (L-NMMA), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA) [139]
