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Mice were monitored for health daily and cages were changed biweekly

Table 1 Common Fusion Tags and Their Properties Tag Hexa-histidine Size (kDa) 0.84 Ligand Ni-IDA Elution Imidazole Properties Small size and easy to integrate into protein, simple purification process, mild elution condition Tag GST Size (kDa) 26 Ligand Glutathione Elution Glutathione Properties Simple purification process, mild elution condition Tag Biotin Size (kDa) 0.244 Ligand Monomeric avidin Elution Biotin Properties Small size, high specificity and affinity to avidin, simple purification process, mild elution condition Size (kDa) Ligand Elution Properties Hexa-histidine 0.84 Ni-IDA Imidazole Small size and easy to integrate into protein, simple purification process, mild elution condition GST 26 Glutathione Glutathione Simple purification process, mild elution condition Biotin 0.244 Monomeric avidin Biotin Small size, high specificity and affinity to avidin, simple purification process, mild elution condition A polyhistidine tag, also called 6xHis-tag, His-tagged, and His-tag is a versatile tool for purifying the highly purified recombinant protein from various expression systems, including bacterial, yeast, plant cell, and mammalian cells systems

It plays an important role in stimulating GLP-1 and satiety at a brain-body level

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RT volume was determined for the 8 weeks for the upper- and lower-body exercises, and was calculated as the number of sets x the number of repetitions x weight/set x the number of training sessions

These cases benefit from more individualized care, potentially including hormone panel monitoring and personalized dosing strategies that go beyond what Brello standard protocol offers