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Cloning and site-directed mutagenesis All plasmids and primers for cloning and site-directed mutagenesis are listed as separate Supplementary Data

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Additionally, stress-inducing agents such as tunicamycin, thapsigargin and histidinol have been shown to modulate CHAC1 regulation, indicating its involvement not only during ER stress but also in the context of the ISR ( CHAC1 promoter is the primary mechanism for the transcriptional upregulation of the gene

This increased blood supply accelerates the delivery of oxygen and nutrients to damaged areas, supporting faster healing