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Control for vehicle effects: Match DMSO or other vehicle concentration across all groups and validate vehicle tolerance in the specific assay system

People with Digestive Disorders: Conditions like Crohns disease, celiac disease, or those who have undergone weight loss surgery can impair the absorption of B12

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This step is consistently cited as the contamination-prevention checkpoint

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Chang, C., Tsai, W., Hsu, Y., & Pang, J