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Quantitative real-time PCR (qRT-PCR) was conducted using the ABI Viia 7 Real-Time PCR System (Applied Biosystems, USA) with ChamQ SYBR qPCR Master Mix (Vazyme, China)

Approximately 454 of 1431 upregulated genes in Trim37 ko3 PGCs exhibited open chromatin regions and increased H3K27ac signals in their regulatory regions (Fig

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However, its important to note that this effect is not universal, and some people may not experience any increase in headache frequency or severity

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In a related study, Zhao et al