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The percentage of 5-mC was calculated using the second-order regression equation of the standard curve constructed with the negative and positive controls

As a result of these shocks, the rat would become agitated and attack the second rat

Thus, the term neuroprogression is used to denote the progressive changes that BD presents from the initial stages to the more advanced stages of the disease, relating the severity of BD to the loss of response to treatment and the number of previous episodes

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