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Samples were then passed through nylon filters with a pore size of 70 m (BD Falcon, Franklin Lakes, NJ, USA), followed by centrifugation at 400 g and 4 C for 5 minutes

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A study revealed that L-methionine supplementation (1.7 g/kg/day, P.O for 8 weeks) was sufficient to produce hyperhomocysteinemia in rats and markedly increased the mean arterial pressure, oxidative stress and mean cardiomyocyte diameter [107]

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