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One more wash 3 5 min in Tris-HCl 50 mM (pH=7.5) at RT 4 mL/well (12-well plate) was done before mounting the slices on gelatine coated slides (adding Prolong Gold antifade mounting medium, Invitrogen, P36930)

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The stress of a new diet or exercise routine can also destabilize mood, and hormonal changes associated with weight loss, which can contribute to mood swings, irritability, and emotional lability

The experimental protocols were consistent with the guidelines issued by the U.S

The GLP-1 Nausea Timeline: Why Nausea Often Fluctuates Nausea is one of the most recognizable GLP-1 side effects