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Functional approaches typically prioritize diet, gut health, and other supportive therapies first, using chelation only in carefully monitored situations

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The number of NG neurons labeled with Alexa 488-CTB retrogradely transported from the stomach were nearly equal (18.2% of 566 left NG neurons and 16.7% of 479 right NG neurons) (Fig

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Avoid repeated freeze-thaw cycling, and verify concentration by UV absorbance at 280 nm where assay design requires it, since the single tryptophan residue gives a usable chromophore