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With 10 mL of water in a 2 mg vial, you might only get 4-5 usable doses before the liquid level drops too low to draw from effectively

Look for these warning signs: any unexpected color change (especially yellow, brown, or amber), cloudiness or haziness, visible particles or debris floating in the solution, or a broken seal on the pen or vial

Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA)

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