Measure drivers: iron, lipid peroxides (MDA), phospholipids Measure defences: GPX activity, total and reduced glutathione, GST Profile context: cholesterol, amino acids and TCA metabolites Building a ferroptosis read-out A practical way to combine assays into a complete picture of the pathway
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In addition to their well-characterized catalytic activity, there is evidence that GST isoenzymes also participate in regulating the expression of mitogen-activated protein kinases, and promote S-glutathionylation of cysteine residues in target proteins (19)
doi: 10.1089/thy.2012.0548 81 LiuMSongJJiangYLiuYPengJLiangHet al