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Following treatment, the medium was removed, cells were washed 2 times with warm PBS, and fixed with 4% formaldehyde for 20 min

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During treatment of the PDX model of osteosarcoma, since the individuality of the mice, the tumor volume of one mouse reached 1500 mm 3 by the end of the treatment, and this mouse was not euthanized in time to obtain reliable and intuitive experimental results

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