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Cells were then fixed with 4% cold (4 C) paraformaldehyde in cytoskeleton buffer for 10 min at 4 C, permeabilized in 0.5% Triton X-100 in warm (37 C) cytoskeleton buffer at room temperature, washed twice with PBS at room temperature, and immunostained

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This product is supplied strictly for laboratory research and must not be used for human consumption or veterinary applications

Oxidative stress occurs when there is an imbalance between excessive reactive oxygen species (ROS) production and/or diminished cellular antioxidant defenses

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Most users add 2 mL BAC water to an 80mg vial (40 mg/mL concentration) and get ~4 weeks of loading dosing, which fits inside the window