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Notably, compensatory crosstalk may limit monotherapy efficacymelanoma cells escaping ferroptosis via FSP1 upregulation may become more dependent on necroptosis suppression, and vice versaunderscoring the rationale for co-targeting multiple pathways to avoid adaptive resistance ( 5 Synergistic therapeutic strategies targeting RCD pathways and immune checkpoint inhibitors Regulated cell death (RCD) pathways, including ferroptosis, pyroptosis, and necroptosis, have emerged as crucial modulators of antitumor immunity, serving as a bridge between tumor cell elimination and immune response activation (123)

Ma, W., Berg, J

We then measured 0.3 l of each sample using a 1 l Hamilton syringe (Hamilton, 80135) and extracted these volumes in 20 l of a 75:25:0.1 HPLC grade acetonitrilemethanolformic acid extraction mix with the following labelled stable isotope internal standards: 2 H 9 -choline (DLM-549), 13 C 4 -3-hydroxybutyrate (CLM-3853), 13 C 6 , 15 N 2 -cystine (CNLM-4244), 13 C 3 -lactate (485926, Sigma-Aldrich), 13 C 6 -glucose (CLM-1396), 13 C 3 -serine (CLM-1574), 13 C 2 -glycine (CLM-1017), 13 C 5 -hypoxanthine (CLM-8042), 13 C 2 , 15 N-taurine (CNLM-10253), 13 C 3 -glycerol (CLM-1510), 2 H 3 -creatinine (DLM-3653), 13 C 10 -kynurenic acid (80445, Sigma-Aldrich), 13 C 10 , 15 N 5 -adenosine (CNLM-3806), 13 C 8 -indole-3-carboxaldehyde (CLM-10745), 15 N 4 -inosine (NLM-4264), 13 C 10 -kynurenine (CLM-9884) and 13 C 4 -methylmalonic acid (CLM-9426)

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Additionally, RANKL emerged as a potential link between DPP-4 and bone-energy metabolism, as it induced DPP-4 expression in osteoclasts, leading to decreased GLP-1 levels and increased blood glucose (Fig