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and (2) assessing the response to a 50 ms pre-pulse (16 kHz pure tone, 70 dB SPL) in a quiet environment, also presented 100 ms before the startle pulse (110 dB SPL, 50 ms duration), which reflects pre-pulse inhibition (PPI) of the acoustic startle (Fig

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Consistency is more important than the specific hour, so choose a time that fits easily into your daily routine