2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C
Some intact peptide and active fragments reach the intestinal mucosa
Most people meet these needs through diet alone, but older adults, people on strict plant-based diets, and those with digestive disorders may benefit from supplements, though dosages vary based on individual needs
Animal studies show no toxicity up to high doses, but human reports are anecdotal
Have asthma
D., Crocetti, D., Tommerdahl, M., Mostofsky, S