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Our bodies naturally produce Vitamin D3 when exposed to sunlight, but with limited sun exposure, it can be challenging to maintain optimal levels

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Then, the sections were washed with 0.1 M PB, endothelial cells were counterstained with lectin, and nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI, Invitrogen), according to the manufacturers protocols

You can typically dispose of it in your regular household trash

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