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For colony formation assays, cells (5 10 4 ) were suspended in 2 mL of 0.33% noble agar (Difco) and laid over 6-well culture plates previously coated with 2 mL of 0.5% noble agar

References 1 NIH Office of Dietary Supplements CholineFact sheet for health professionals: cholines role in VLDL export and hepatic fat metabolism, deficiency-related steatosis, and high-intake side effects such as fishy odor and hypotension

J Rheumatol 9(4):519526 Gurer H, Ozgunes H, Neal R, Spitz DR, Ercal N (1998) Antioxidant effects of N-acetylcysteine and succimer in red blood cells from lead-exposed rats