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The mechanism is thought to involve binding of the peptide C terminus to the receptor extracellular domain, followed by insertion of the peptide N terminus into the pocket formed by the transmembrane helices and extracellular loops, instigating receptor activation and signalling 19

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Cytosolic fraction was clicked directly, whereas for the membrane fraction, the supernatant was fully removed, and the pellet was washed once with 200 l PBS before resuspension in 200 l 0.4% SDSPBS

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