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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

296 In addition, in the further study with tumor-bearing mice models, macrophages from mice of different ages exist diverse phenotypes, indicating that aging is accompanied with changes in inflammatory cells and will affect tumor progression

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Social isolation in rats inhibits oxidative metabolism, decreases the content of mitochondrial K-Ras and activates mitochondrial hexokinase (in Eng)