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After pancreas digestion and separation of pancreatic islets in a polysucrose density gradient medium, islets were purified by handpicking under a macroscope and were cultured during 16 hours before experiments
However, it is significantly more expensive per milligram than lyophilized vials and is locked to fixed dose increments
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10.1080/10715762.2018.1468564 4 ArakakiN.YamashitaA.NiimiS.YamazakiT
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