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investigated rapamycin pharmacokinetics following a single oral dose of 0.1 mg/kg or a five-day course of this treatment.[51] In the single-dose protocol, rapamycin concentration peaked twice, at two- and six-hours post-administration, but this pattern was not noted when the dogs were treated on five consecutive days
After a wash step, a horseradish peroxidase (HRP) conjugated murine anti-AMG 479, clone B35 at 400 ng/mL was added to the plate and incubated for 1.5 h
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