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The culture medium was Dulbecco's Modified Eagle Medium (DMEM, D5796), supplemented with 10% fetal bovine serum (FBS, F7524), and 100 U/mL Penicillin-Streptomycin (P4333), referred to as complete DMEM. For HT29-MTX cells, 1% MEM Non-essential Amino Acid Solution (M7145,) was also added

Let it reach room temperature naturally

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The dominance of Ruminococcaceae_bacterium GD6 in this genus has been reflected in related studies on ulcerative colitis (Xie et al., 2023)

Every mouse study showed longer life. Some models reported lifespan changes

Leading many organizations to question and evaluate whether GLP-1s are a worthwhile investment for their populaiton