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Apply every other day for the first two weeks

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Heat, repeated freeze-thaw cycles, and vigorous shaking promote aggregation and denaturation

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Freeze-thaw cycling can disrupt the copper coordination sphere as well as degrade the peptide backbone, so single-use aliquots in amber or foil-wrapped vessels are preferable to repeated access of one stock

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Longer needles become unnecessary since BPC-157 subcutaneous injection does not require deep penetration

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