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After 6-hour incubation, the ROS Assay Kit -Highly Sensitive DCFH-DA-Reagent (Dojindo Labratories) were employed for detection

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8 weeks (3 vials): 9 mL total 1 10 mL bottle 12 weeks (6 vials): 18 mL total 2 10 mL bottles 16 weeks (10 vials): 30 mL total 3 10 mL bottles Alcohol Swabs Use two swabs daily one for sterilizing the vial and one for the injection site