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Purity: 99.42 % Stage-3: Synthesis of Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH [fragment- 3] Step-A: CTC resin (50 grams) was taken in a SPPS reactor and dichloromethane was added and allowed it to swell for 10 minutes